Biology Laboratory Calculators
Laboratory calculators for molecular biology and microbiology — DNA and protein concentration, cell dilution and doubling time, ligation, qPCR efficiency, and log reduction.
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DNA Concentration Calculator
Calculate DNA concentration from amount and volume, A260 absorbance, dilution (C1V1), total DNA, and pipette volume — with ng/µL and µg/mL units.
Protein Concentration Calculator
Calculate protein concentration from mass/volume, A280 Beer–Lambert, C1V1 dilution, or Bradford/BCA/Lowry standard curves — mg/mL and µg/mL.
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Annealing Temperature Calculator
Estimate PCR primer annealing temperature (Tm) from a DNA sequence or GC content.
Bacteria Generation Time Calculator
Calculate bacterial generation time from initial and final counts over a period.
Cell Dilution Calculator
Solve C1V1 = C2V2 dilutions for cells, DNA, and reagents in the lab.
Cell Doubling Time Calculator
Calculate cell population doubling time from initial and final cell counts.
DNA Concentration Calculator
Calculate DNA concentration from amount and volume, A260 absorbance, dilution (C1V1), total DNA, and pipette volume — with ng/µL and µg/mL units.
Ligation Calculator
Calculate insert:vector molar ratio and recommended volumes for DNA ligation.
Log Reduction Calculator
Calculate log reduction of microbes from initial and final counts for disinfection.
Protein Concentration Calculator
Calculate protein concentration from mass/volume, A280 Beer–Lambert, C1V1 dilution, or Bradford/BCA/Lowry standard curves — mg/mL and µg/mL.
Frequently asked questions
A:By absorbance at 260 nm: 1 A₂₆₀ = 50 µg/mL double-stranded DNA, using the Beer–Lambert law.
A:The dilution equation: initial concentration × volume equals final concentration × volume.
A:Between 90% and 110% (slope ≈ −3.1 to −3.6), per MIQE guidelines.